亚剂量硫酸新霉素对黄羽肉鸡回肠黏膜菌群组成的影响

doi:10.11843/j.issn.0366-6964.2016.11.019

Abstract

Abstract:

The experiment was conducted to study on the effects of subclinical doses of neomycin sulfate on the ileal mucosa-associated microbiota (MAM) composition and dynamic change of broiler chickens at 1 to 42 days old.Chicken ileal segments were collected in group A (basal diet) and B (basal diet with 50 mg•kg^-1 Neomycin Sulfate)，and MAM DNA were extracted at day 1，7，14，21，28，35 and 42，respectively.The community of the ileal MAM between two groups were analyzed by Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE)at various ages，and the random clone library of 16S rDNA V3 region of the MAM in ileum at 42 days old between Group A and D was established by analysis of 16S rDNA gene sequences.DGGE profiles showed that the structure of the MAM in ileum between the two groups were of remarkable difference during day 1-28，and the similarity of the MAM in ileum was 40%-60%.The alterations of MAM of ileum were remarkably different between the two groups during day 1-35，with 9%-33% change in group A and 17%-45% change in group D.Analysis of the library in ileum indicated that nine operational taxonomic units (OTUs) (form 94 gene sequences or clones) were detected in the group A，as compared with 6 OTUs (form 93 clones) in the group D.Exiguobacterium and Lactobacillus were predominant in the group A and D，counting for 48.94%，38.30% and 45.16%，49.46%， respectively.The amount of other species found in ileum in group A were different from that in group D.In addition，sequence known being related to Colibacillus were not detected in group A and D.These results indicated that the composition and diversity of the MAM in ileum at day 42 does exist difference between group A and D，especially the proportion of Lactobacillus，these difference might be caused by the continuous antibiotic pressure selection.

CLC Number:

S852.6

RONG Ting，LIU Zhi-chang，WANG Gang，LI Shu-hong，ZHANG Jie，CAI Zhui，CHEN Zhuang. Impact of Subclinical Doses of Neomycin Sulfate on the Ileal Mucosa-Associated Microbiota Composition of Broiler Chickens[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(11): 2301-2309.

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References

［1］WASKMAN S A，LECHEVALIER H A.Neomycin，a new antibiotic active against streptomycin-resistant bacteria，including tuberculosis organisms［J］.Science，1949，109(2830)：305-307.
［2］CLAROT I，REGAZZETI A，AUZEIL N，et al.Analysis of neomycin sulfate and framycetin sulfate by high-performance liquid chromatography using evaporative light scattering detection［J］.J Chromatogra A，2005，1087：236-244.
［3］THONGSRISOMBOON P，LIAWRUANGRATH B，LIAWRUANGRATH S，et al.Flow injection chemiluminescence determination of Neomycin in pharmaceutical formulations［J］.J Flow Injection Anal，2010，27(1)：36-41.
［4］ISOHERRANEN N，SOBACK S.Determination of gentamicins C1，C1a，and C2 in plasma and urine by HPLC［J］.Clin Chem，2000，46(6)：837-842.
［5］HUYGHEBAERT G，DUCATELLE R，IMMERSEEL F V.An update on alternatives to antimicrobial growth promoters for broilers［J］.Vet J，2011，187(2)：182-188.
［6］DIBNER J J，RICHARDS J D.Antibiotic growth promotersin agaculture：history and mode of action［J］.Poult Sci，2005，84(4)：634-643.
［7］LI M，GONG J H，COTTRILL M，et al.Evaluation of QIAampR@ DNA Stool Mini Kit for ecological studies of gut microbiota［J］.J Microbiol Meth，2002，54(1)：13-20.
［8］张洁，容庭，刘志昌，等.两纯系鸡胃肠道黏膜细菌l6S rDNA的PCR-DGGE分析比较［J］.畜牧兽医学报，2013，44（2）：204-210.
ZHANG J，RONG T，LIU Z C，et al.Comparative analysis of 16S rDNA of the gastrointestinal mucosa-associated microbiota between two chicken pure lines by PCR-DGGE［J］.Acta Veterinaria et Zootechnica Sinica，2013，44(2)：204-210.(in Chinese)
［9］容庭，张洁，王刚，等.16S rDNA克隆文库方法分析两纯系鸡回肠及盲肠细菌的组成［J］.畜牧兽医学报，2014，45（8）：1218-1227.
RONG T， ZHANG J， WANG G， et al. Analysis of composition of the mucosa-associated microbiota in ileum and cecum between two chicken pure lines by 16S rDNA clone library［J］.Acta Veterinaria et Zootechnica Sinica, 2014,45(8):1218-1227.(in Chinese)
［10］MUYZER G，DE WAAL E C，UITTERLINDEN A G.Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S Rrna［J］.Appl Environ Microbiol，1993，59(3)：695-700.
［11］MARZORATI M，WITTEBOLLE L，BOON N，et al.How to get more out of molecular fingerprints：practical tools for microbial ecology［J］.Environ Microbiol，2008，10(6)，1571-1581.
［12］GOOD I L.The population frequencies of species and the estimation of population parameters［J］.Biometrika，1953，40：237-264.
［13］ELISHAHED M S，SENKO J M，NAJAR F Z，et al.Bacterial diversity and sulfur cycling in a mesophilic sulfide-rich spring［J］.Appl Environ Microbiol，2003，69(9)：5609-5621.
［14］HILL T C J，WALSH K A，HARRIS J A，et al.Using ecological diversity measures with bacterial communities［J］.FEMS Microbiol Ecol，2003，43(1)：1-11.
［15］SAVAGE D C.Microbial ecology of the gastrointestinal tract［J］.Annu Rev Microbiol，1977，31(70)：107-133.
［16］ZOETENDAL E G，COLLIER C T，KOIKE S，et al.Molecular ecological analysis of the gastrointestinal microbiota：A review［J］.J Nutr，2004，134(2)：465-472.
［17］ZHU X Y，ZHONG T，PANDYA Y，et al.16SrRNA-based analysis of microbiota from the cecum of broiler chickens［J］.Appl Environ Microbiol，2002，68(1)：124-137.
［18］ZOETENDAL E G，VON WRIGHT A，VILPPONEN-SALMELA T，et al.Mucosa-associated bacteria in the human gastrointestinal tract are uniformly distributed along the colon and differ from the community recovered from feces［J］.Appl Environ Microbiol， 2002，68(7)：3401-3407.
［19］LEPAGE P，SEKSIK P，SUTREN M，et al.Biodiversity of the mucosa-associated microbiota is stable along the distaldigestive tract in healthy individuals and patients with IBD［J］. Inflamm Bowel Dis， 2005，11(5)：473-480.
［20］KNARREBORG A，SIMON M A，ENGBERG RMJENSEN B B，et al.Effects of dietary fat source and subtherapeutic levels of antibiotic on the bacterial community in the ileum of broiler chickens at various ages［J］.Appl Environ Microbiol，2002，68(12)：5918-5924.
［21］SMIRNOV A，PEREZ R，AMIT-ROMACH E，et al.Mucin dynamics and microbial populations in chicken small intestine are changed by dietary probiotic and antibiotic growth promoter supplementation［J］.J Nutr，2005，135(2)：187-192.
［22］PEDROSO A A，MENTEN J F M，LAMBAIS M R，et al.Intestinal bacterial community and growth performance of chickens fed diets containing antibiotics［J］.Poult Sci，2006，85(4)：747-752.
［23］DUMONCEAUX T J，HILL J E，SEAN M，et al.Characterization of intestinal microbiota and response to dietary virginiamycin supplementation in the broiler chicken［J］.Appl Environ Microbiol，2006，72(4)：2815-2823.
［24］ZHOU H，GONG J，BRISBIN J T，et al.Appropriate chicken sample size for identifying the composition of broiler intestinal microbiota affected by dietary antibiotics，using the Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis Technique［J］. Poult Sci，2007，86(12)：2541-2549.
［25］JOHANSEN C H，BJERRUM L, PEDERSEN K.Impact of salinomycin on the intestinal microflora of broiler chickens［J］.Acta Vet Scand，2007，49(1)：30.
［26］VAN HEMERT S，HOEKMAN A J，SMITS M A，et al.Gene expression response to a Salmonella infection in the chicken intestine differ between lines［J］.Vet Immunol Immunopathol， 2006，114(3-4)：247-258.
［27］BJERRUM L，PEDERSEN K，ENGBERG R M.The influence of whole wheat feeding on Salmonella infection and gut flora composition in broilers［J］.Avian Dis，2005，49(1)：9-15.
［28］LUMPKINGS B S，BATAL A B，LEET M D.Evaluation of the bacterial community and intestinal development of different genetic lines of chickens［J］.Poult Sci，2010，89(8)：1614-1621.
［29］GONG J H，FORSTER R J，YU H，et al.Molecular analysis of bacterial populations in the ileum of broiler chickens and comparison with bacteria in the cecum［J］.FEMS Microbiol Ecol，2002，41(3)：171-179.
［30］LEY R E，BÄCKHED F，TURNBAUGH P，et al.Obesity alters gut microbial ecology［J］.Proc Natl Acad Sci U S A，2005，102(31)：11070-11075.

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Impact of Subclinical Doses of Neomycin Sulfate on the Ileal Mucosa-Associated Microbiota Composition of Broiler Chickens

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